| Customization: | Available |
|---|---|
| Powder: | Yes |
| Customized: | Non-Customized |
Suppliers with verified business licenses
Audited Supplier Audited by an independent third-party inspection agency
CAS No.: 39450-01-6
EINECS No.: 3.4.21.64
Form: Lyophilized White Powder.
Source: Mutated gene from Tritirachium limber, Expressed in recombinant yeast.
Specific Activity: ≥34U/mg dry weights
Package: 100mg/Bottle, 1g/Bottle (Standard) or 1-200 gram Customer packing.
Storage Conditions:Store at 4 to -20ºC recommended.
Mutated Proteinase K powder is stable at room temperature in 3 years.
Expiration : Three years if kept below 4ºC.
Molecular Weight: 29,300 D
QC Assays:
Activity Assay and Unit Definitions:
One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37º C, pH7.5.
DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37ºC.
RNase Activity: none detectable of ribnuclease activity after 16 hours incubation at 25 º C
Protein Purity: over 99% (Native-PAGE and SDS-PAGE assay)
|
Description |
White powder |
Complies |
|
Identification |
IR |
Complies |
|
|
HPLC |
Complies |
|
Heavy metal |
≤10ppm |
5ppm |
|
Pb |
≤3ppm |
1.5ppm |
|
Hg |
≤0.1ppm |
0.05ppm |
|
Cd |
≤1ppm |
0.2ppm |
|
Loss on drying |
≤0.5% |
0.12 |
|
Residue on lgnition |
≤0.1% |
0.03 |
|
Single impurity |
≤0.5% |
0.12 |
|
Total impurity |
≤1.0% |
0.29 |
|
Total bacteria |
≤1000cfu/g |
<1000 |
|
Yeast and Mould |
≤100 cfu/g |
<000 |
|
E.coli/25g |
Absent |
Absent |
|
Salmonella/25g |
Absent |
Absent |
|
Assay |
≥99.0% |
99.4% |
|
Conclusion |
Conforms with USP/EP standards |
|
The application of this Mutated Proteinase K is similar with wild type Proteinase K. But the mutated one has higher specific activity and more stable at RT. It is a broad substrate non-specific serine Proteinase. It is active over the pH range 4-11. It was used on any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies.Mutated Proteinase K is active with SDS, urea and EDTA and active between the temperature15-75ºC.
Now,Proteinase K is a broad substrate non-specific serine proteinase. It was used on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from different kinds of cells.
It's been used on glycoprotein modification and protein structure studies also. Proteinase K is active with SDS, urea and EDTA.
Proteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.
Adding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.
Recently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.
Proteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces.
Because of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.
Qingdao Sigma Chemical Co.,Ltd is a global chemical industry manufacturers and suppliers of pharmaceuticals and intermediates, food and feed additives, herbal extracts, agrochemicals and fine chemicals etc.
The aim of Qingdao Sigma Chemical Co.,Ltd is a quality policy that "Elements in the system all have been satisfied, Personnel has been trained; Every batch of material is checked, Each procedure guarantees the quality; Every bag of our products is top grade, Service is thoughtful all the time, quality and reputation to increase market share and promote enterprise development. The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
){kind=link}